S&M Young Researcher Paper Award 2020
Recipients: Ding Jiao, Zao Ni, Jiachou Wang, and Xinxin Li [Winner's comments]
Paper: High Fill Factor Array of Piezoelectric Micromachined
Ultrasonic Transducers with Large Quality Factor

S&M Young Researcher Paper Award 2021
Award Criteria
Notice of retraction
Vol. 32, No. 8(2), S&M2292

Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.

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Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification

Ryo Ishii, Kazuhiro Morioka, Takuya Mizumoto, Natsumi Yamasaki, Akihide Hemmi, Atsushi Shoji, Hiroya Murakami, Norio Teshima, Tomonari Umemura, Katsumi Uchiyama, and Hizuru Nakajima

(Received September 3, 2021; Accepted November 18, 2021)

Keywords: fluorescence microplate reader, LAMP, ITO glass heater, on-site nucleic acid test, variety discrimination

We developed a portable fluorescence microplate reader equipped with an indium tin oxide (ITO) glass heater for nucleic acid testing based on loop-mediated isothermal amplification (LAMP). The components used for detection (e.g., LEDs, optical filters, the microwell plate, the ITO glass heater, and photodiodes) are arranged in parallel on a straight line. This optical placement allows us to simultaneously heat nine sample solutions and monitor the fluorescence intensity amplified by the LAMP reaction despite the analysis instrument being handheld. This simultaneous monitoring was demonstrated by the LAMP reaction using positive and negative controls. In addition, the microplate reader was applied to the variety discrimination of rice, and Koshihikari rice was successfully discriminated from four types of milled rice using the microplate reader. The developed microplate reader will be useful for on-site nucleic acid testing because it can be operated using batteries and is portable.

Corresponding author: Kazuhiro Morioka, Hizuru Nakajima




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