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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 31, Number 5(2) (2019)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 1647-1655
S&M1887 Research Paper of Special Issue https://doi.org/10.18494/SAM.2019.2285 Published: May 31, 2019 Real-time Polymerase Chain Reaction System Using a Camera for Open Platform [PDF] Ji-Soo Hwang, Chan-Young Park, Hye-Jeong Song, Yu-Seop Kim, Jongwon Kim, and Jong-Dae Kim (Received April 16, 2018; Accepted March 20, 2019) Keywords: fluorescence detection, USB camera, real-time polymerase chain reaction, open platform
In real-time polymerase chain reaction (RT-PCR), the result of the amplification of DNA can be seen through changes in fluorescence brightness sensed during the PCR cycles. To detect the changes in fluorescence intensities, commercial real-time PCR thermocyclers mainly use photodiodes for detection. However, our previous study showed that a digital single lens reflex (DSLR) camera instead of a photodiode can detect changes in fluorescence intensity as well as differentiate cases of unsatisfactory experimental results. Although the fluorescence detection was successful, the adoption of the DSLR camera was problematic in terms of size and cost. In this paper, we show that fluorescence detection is also possible using a small USB camera for open platforms instead of bulky DSLR cameras.
Corresponding author: Jong-Dae Kim  This work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Ji-Soo Hwang, Chan-Young Park, Hye-Jeong Song, Yu-Seop Kim, Jongwon Kim, and Jong-Dae Kim, Real-time Polymerase Chain Reaction System Using a Camera for Open Platform, Sens. Mater., Vol. 31, No. 5, 2019, p. 1647-1655. |
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- A Prototype Portable Voltammetric Sensor for Determining Titratable Acidity of Sake and Moromi
Akira Kotani, Kokoro Taniguchi, Koichi Machida, Kazuhiro Yamamoto, and Hideki Hakamata - PACKTEST for L-Glutamate Quantification: Development of On-site and High-throughput Analytical Kits using L-Glutamate Oxidase Mutant
Keita Murai, Hiroki Yamaguchi, Satoru Furuuchi, Kazutoshi Takahashi, Uno Tagami, Moemi Tatsumi, Toshimi Mizukoshi, Hiroshi Miyano, Shuntaro Okauchi, and Masayuki Sugiki - Development of Portable Multi-fluorescence Detection System Using Indium Tin Oxide Heater for Loop-mediated Isothermal Amplification
Ryo Ishii, Sota Hirose, Shoji Yamamoto, Kazuhiro Morioka, Akihide Hemmi, and Hizuru Nakajima - Improved Substrate Specificity of Phenylalanine Dehydrogenase for L-Phenylalanine Sensor
Moemi Tatsumi, Kazutoshi Takahashi, Hiroki Yamaguchi, Uno Tagami, Hiroshi Miyano, Toshimi Mizukoshi, and Masayuki Sugiki - Reproduction of Absorption Spectra of Bromothymol Blue–Methyl Red Mixed Indicator from RGB and L*a*b* Color Coordinates and Application to Fast Spectrum Acquisition
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