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Notice of retraction
Vol. 34, No. 8(3), S&M3042

Notice of retraction
Vol. 32, No. 8(2), S&M2292

Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials, Volume 36, Number 3(1) (2024)
Copyright(C) MYU K.K.
pp. 811-822
S&M3563 Research Paper of Special Issue
https://doi.org/10.18494/SAM4811
Published: March 8, 2024

Development of Monoclonal Antibodies and Immunochromatographic Test Strips Applying Indirect Competitive Method for Daidzein Detection [PDF]

Takeshi Onodera, Taiki Ajisaka, Ayako Ogawa, Yuko Hashiguchi, Nozomu Sakurai, and Akifumi Sugiyama

(Received November 30, 2023; Accepted January 10, 2024)

Keywords: daidzein, immunochromatography, surface plasmon resonance, indirect competitive method, monoclonal antibody

In this study, we aimed to detect daidzein secreted in the soybean rhizosphere using an indirect competitive immunochromatographic assay. A specific anti-daidzein monoclonal antibody was obtained through the establishment of a hybridoma. The antibody exhibited a detection limit of 5 ppb daidzein and showed strong cross-reactivity with daidzein, as determined by an indirect competitive method using a surface plasmon resonance sensor. Through the findings, a unique binding pattern differing from those of genistein and other saponins was observed. Enhanced sensitivity with the ability to detect daidzein concentrations as low as 10 nM (2.5 ng/mL) was achieved using immunochromatographic test strips with modified gold nanoparticles

Corresponding author: Takeshi Onodera


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This work is licensed under a Creative Commons Attribution 4.0 International License.

Cite this article
Takeshi Onodera, Taiki Ajisaka, Ayako Ogawa, Yuko Hashiguchi, Nozomu Sakurai, and Akifumi Sugiyama, Development of Monoclonal Antibodies and Immunochromatographic Test Strips Applying Indirect Competitive Method for Daidzein Detection, Sens. Mater., Vol. 36, No. 3, 2024, p. 811-822.



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