pp. 4769-4779
S&M3429 Research Paper of Special Issue https://doi.org/10.18494/SAM4473 Published: October 25, 2023 Enzyme-immobilized Screen-printed Sensor for One Drop of Sample Using Porous Silica Spheres [PDF] Shunsuke Fujii, Akiko Yoshida, Tracy T Chuong, Yuka Minegishi, Yuta Nishina, Hirotomo Nishihara, Kouji Masumoto, and Tetsuji Itoh (Received April 27, 2023; Accepted August 24, 2023) Keywords: biosensor, enzyme electrode, mediator, screen-printed device, porous silica
A sensor chip that can detect enzyme electrode reactions with a sample volume as small as 20 μL has been developed using screen-printing technology with porous silica spheres (PSSs). PSSs are spherical particles of silica with mesoporous pores, a material with a large specific surface area. Other material properties of PSSs include good dispersibility, high mechanical strength, and the ability to be synthesized with precisely controlled mesopore sizes. These advantages support the increased adsorption of enzymes to create highly sensitive sensors. The electrode was designed as follows: carbon paste (CP) with PSSs was used as the working electrode, glucose dehydrogenase (GDH) was used as the enzyme, and quinoline-5,8-dione (QD) was used as the mediator of electron transfer to induce a glucose dehydrogenation reaction. The sensitivity of the sensor was demonstrated through the detection of glucose. The glucose sensor showed reliable sensitivity between 1 and 60 mM glucose, which is comparable to the detection range of a traditional working electrode such as platinum. Furthermore, the GDH had minimal reduction in activity over repeated usage. This device demonstrates the ability to create an equally sensitive but less expensive and reusable sensor that has the potential to better suit general applications.
Corresponding author: Kouji Masumoto and Tetsuji ItohThis work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Shunsuke Fujii, Akiko Yoshida, Tracy T Chuong, Yuka Minegishi, Yuta Nishina, Hirotomo Nishihara, Kouji Masumoto, and Tetsuji Itoh, Enzyme-immobilized Screen-printed Sensor for One Drop of Sample Using Porous Silica Spheres, Sens. Mater., Vol. 35, No. 10, 2023, p. 4769-4779. |