pp. 4761-4768
S&M3428 Research Paper of Special Issue https://doi.org/10.18494/SAM4432 Published: October 25, 2023 Development of Organic–Inorganic Nanostructure Labels for Food-Poisoning Bacteria [PDF] Shogo Nakamura, Akihiro Nakao, Satohiro Itagaki, Kyohei Matsui, Shigeki Nishii, Xueling Shan, Yojiro Yamamoto, Yasuhiro Sadanaga, Zhidong Chen and Hiroshi Shiigi (Received April 17, 2023; Accepted September 22, 2023) Keywords: Staphylococcus aureus, copper nanostructures, electrochemical labels, organic–inorganic hybrids, food-poisoning bacteria
In this paper, we describe a simple strategy to detect food-poisoning bacteria using the spectroscopic and electrochemical properties of organic–inorganic nanostructures composed of electrically conductive polyaniline-coated copper nanoparticles. Antibody-introduced nanostructures specifically bind to cells via antigens and function as nanometer-sized labels. Attempting the quantification of Staphylococcus aureus using nanostructure labels, we found that the absorbance of the free label in the supernatant decreased as the number of bacterial cells increased, indicating that the labeled cells were obtained as precipitates. Furthermore, the peak current (Ip) based on the number of labels bound to cells on the electrode increased as the number of bacterial cells increased. Thus, the introduction of antibodies into nanostructures has made it possible to electrochemically and spectroscopically quantify the causative bacteria of food poisoning.
Corresponding author: Hiroshi ShiigiThis work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Shogo Nakamura, Akihiro Nakao, Satohiro Itagaki, Kyohei Matsui, Shigeki Nishii, Xueling Shan, Yojiro Yamamoto, Yasuhiro Sadanaga, Zhidong Chen and Hiroshi Shiigi, Development of Organic–Inorganic Nanostructure Labels for Food-Poisoning Bacteria, Sens. Mater., Vol. 35, No. 10, 2023, p. 4761-4768. |